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(A) Visualization of <t>cholesterol</t> in HCNs and CGNs (as revealed by Filipin staining with counterstaining of neuronal nuclei with TOPRO3. (B) Quantification of Filipin staining in cultured HCNs and CGNs before and after permeabilization with Trion X100. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate, normalized to the CGN group. Results were not significantly different as judged by unpaired t test with Welch’s correction. (C) Quantification of cholesterol levels in HCNs and CGNs using <t>AmplexRed</t> cholesterol detection kit. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate. Results were not significantly different as judged by unpaired t test with Welch’s correction.
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(A) Visualization of <t>cholesterol</t> in HCNs and CGNs (as revealed by Filipin staining with counterstaining of neuronal nuclei with TOPRO3. (B) Quantification of Filipin staining in cultured HCNs and CGNs before and after permeabilization with Trion X100. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate, normalized to the CGN group. Results were not significantly different as judged by unpaired t test with Welch’s correction. (C) Quantification of cholesterol levels in HCNs and CGNs using <t>AmplexRed</t> cholesterol detection kit. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate. Results were not significantly different as judged by unpaired t test with Welch’s correction.
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(A) Visualization of <t>cholesterol</t> in HCNs and CGNs (as revealed by Filipin staining with counterstaining of neuronal nuclei with TOPRO3. (B) Quantification of Filipin staining in cultured HCNs and CGNs before and after permeabilization with Trion X100. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate, normalized to the CGN group. Results were not significantly different as judged by unpaired t test with Welch’s correction. (C) Quantification of cholesterol levels in HCNs and CGNs using <t>AmplexRed</t> cholesterol detection kit. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate. Results were not significantly different as judged by unpaired t test with Welch’s correction.
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(A) Visualization of cholesterol in HCNs and CGNs (as revealed by Filipin staining with counterstaining of neuronal nuclei with TOPRO3. (B) Quantification of Filipin staining in cultured HCNs and CGNs before and after permeabilization with Trion X100. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate, normalized to the CGN group. Results were not significantly different as judged by unpaired t test with Welch’s correction. (C) Quantification of cholesterol levels in HCNs and CGNs using AmplexRed cholesterol detection kit. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate. Results were not significantly different as judged by unpaired t test with Welch’s correction.

Journal: bioRxiv

Article Title: Coupling of death receptor p75 NTR to the RhoA and NF-kB pathways differentially regulates internalization in hippocampal and cerebellar neurons

doi: 10.64898/2026.02.10.705215

Figure Lengend Snippet: (A) Visualization of cholesterol in HCNs and CGNs (as revealed by Filipin staining with counterstaining of neuronal nuclei with TOPRO3. (B) Quantification of Filipin staining in cultured HCNs and CGNs before and after permeabilization with Trion X100. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate, normalized to the CGN group. Results were not significantly different as judged by unpaired t test with Welch’s correction. (C) Quantification of cholesterol levels in HCNs and CGNs using AmplexRed cholesterol detection kit. Results are presented as mean ± SEM of three independent experiments, each performed in duplicate. Results were not significantly different as judged by unpaired t test with Welch’s correction.

Article Snippet: Cholesterol level of cell extraction was detected by AmplexRed cholesterol kit (Invitrogen, A12216).

Techniques: Staining, Cell Culture